Coding

Part:BBa_K4134001:Design

Designed by: Jiankai Liu   Group: iGEM22_Nanjing-China   (2022-09-19)


Atox1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed two short silver-binding peptides(Atox1 and AgBP2) on the C-terminus of the membrane protein to "capture" silver ions. During the bio oxidative electron transfer process, silver ions are reduced and aggregated into silver nanoparticles. The silver short peptides on the membrane protein played the role of enriching silver ions so that the silver nanoparticles (AgNPs) were more concentrated in the outer membrane and periplasmic space. Considering that silver is much more conductive than cytochromes, this approach may help speed up the process of electron transfer and improve cell efficiency.


Source

Homo sapiens antioxidant 1 copper chaperone (ATOX1), mRNA. NCBI Reference Sequence: NM_004045.4


References